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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
04/07/2012 |
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Data da última atualização: |
22/02/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OBREGÓN, D.; OLIVEIRA, M. C. de S.; TIZIOTO, P. C.; FUNNES, M. E.; MARTÍNEZ, S.; ROQUE, E.; FONSECA, A. H.; CORONA, B. |
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Afiliação: |
D. OBREGÓN, Universidad Agraria de La Habana; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE; POLYANA CRISTINE TIZIOTO, UFSCar/SÃO CARLOS, SP; MARIBEL ELIZABETH FUNNES, UFSCar/SÃO CARLOS, SP; SIOMARA MARTÍNEZ, CENSA/MAYABEQUE; E. ROQUE, UNIVERSIDAD AGRARIA DE LA HABANA; A. HENRIQUE FONSECA, UFRRJ/RIO DE JANEIRO; BELKIS CORONA, CENSA/MAYABEQUE. |
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Título: |
Diagnóstico de Babesia bovis en búfalos de la región occidental de Cuba através de un ensayo de nPCR. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Revista de Salud Animal, v. 34, n. 2, p. 1-7, mayo-ago. 2012. |
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Idioma: |
Espanhol |
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Conteúdo: |
The objective of this work was to determine if water buffaloes from the western region of Cuba are carrier of Babesia bovis infection, using a nested PCR (nPCR) test. Two hundred twenty two DNA samples, extracted from buffalo blood at different ages, from six farms in the western region of Cuba were processed. Visualization of blood smear in the optical microcopy was previously carried out. The results showed that all the samples were negative to blood smear; however, 94 (42.1%) samples were positive in the nPCR, visualizing bands of 298 pb. Infection was present in animals from all farms and categories studied. A positive nPCR sample was sequenced and such sequence was deposited in the GenBank, comparing it with others available in diverse databases. Its high identity was verified with several geographical isolates of B. bovis. It is concluded that water buffaloes in the western region of Cuba are carrier of Babesia bovis infection. |
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Palavras-Chave: |
PCRn; Rap1. |
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Thesagro: |
Babesia Bovis. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/61684/1/PROCI-2012.00080.pdf
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Marc: |
LEADER 01644naa a2200241 a 4500
001 1927636
005 2023-02-22
008 2012 bl uuuu u00u1 u #d
100 1 $aOBREGÓN, D.
245 $aDiagnóstico de Babesia bovis en búfalos de la región occidental de Cuba através de un ensayo de nPCR.$h[electronic resource]
260 $c2012
520 $aThe objective of this work was to determine if water buffaloes from the western region of Cuba are carrier of Babesia bovis infection, using a nested PCR (nPCR) test. Two hundred twenty two DNA samples, extracted from buffalo blood at different ages, from six farms in the western region of Cuba were processed. Visualization of blood smear in the optical microcopy was previously carried out. The results showed that all the samples were negative to blood smear; however, 94 (42.1%) samples were positive in the nPCR, visualizing bands of 298 pb. Infection was present in animals from all farms and categories studied. A positive nPCR sample was sequenced and such sequence was deposited in the GenBank, comparing it with others available in diverse databases. Its high identity was verified with several geographical isolates of B. bovis. It is concluded that water buffaloes in the western region of Cuba are carrier of Babesia bovis infection.
650 $aBabesia Bovis
653 $aPCRn
653 $aRap1
700 1 $aOLIVEIRA, M. C. de S.
700 1 $aTIZIOTO, P. C.
700 1 $aFUNNES, M. E.
700 1 $aMARTÍNEZ, S.
700 1 $aROQUE, E.
700 1 $aFONSECA, A. H.
700 1 $aCORONA, B.
773 $tRevista de Salud Animal$gv. 34, n. 2, p. 1-7, mayo-ago. 2012.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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